GDR Micro Nano Fluidique

Mois : février 2015

About CEA-Leti

Leti is an institute of the Commissariat à l’Energie Atomique et aux Energies Alternatives (, a French research-and-technology organization with activities in energy, IT, healthcare, defence and security. Leti is focused on creating value and innovation through technology transfer to its industrial partners. It specializes in nanotechnologies and their applications, from wireless devices and systems, to biology, healthcare and photonics. NEMS and MEMS are at the core of its activities. An anchor of the MINATEC campus, CEA-Leti operates 8,000-m² of state-of-the-art clean room space on 200mm and 300mm wafer platforms. It employs 1,400 scientists and engineers and hosts more than 190 Ph.D. students and 200 assignees from partner companies. CEA-Leti owns more than 1,700 patent families.

For more information, visit

Within the Division of Technology for Biology and Health, the Biosystems on chip Division develops new microfluidic device. In collaboration with Grenoble Hospital, we especially develop new microsystems for pancreatic cells encapsulation. In some case, the only option for people suffering from type I Diabetes is to receive a pancreatic graft. This is associated with long, expensive and restrictive immuno-suppressive treatments. One alternative is to graft encapsulated cells in order to limit or to suppress these immuno-suppressive treatments. Today’s encapsulation technologies involve many manual steps leading to poor reproducibility in batch to batch production. At CEA-Leti we decided to implement a new cell encapsulation system ensuring high quality encapsulation and batch to batch reproducibility.



The core of this project is to implement new functions and new process in the existing cell encapsulation device. In collaboration with other department at CEA, the candidate will have first to develop and characterize a new microfluidic device for cell sorting. These steps involved the set-up of a dedicated bench, the design and conception of microfluidic chips. Experiments on living cell will be performed. Cell viability and functionality will be performed either on primary cell culture or cell lines. In a second time, the candidate will optimize the cell encapsulation process, involving microfluidic tests, cell culture assays, chemical synthesis and chemical surface grafting.



Candidate Profile

The candidate shall speak English (or French) and have good communication skills as he/she will have to work and report inside a collaborative team. He/She shall possess a background in the field of physics/engineering, physics/chemistry, Bioengineering, soft material characterization. Knowledge in microfluidics and/or cell culture would be a plus. The candidate shall have an experimentalist profile and be highly motivated by multidisciplinary project.

Starting date: 1/04/2015

Duration: 12 months, but good prospects for continuing employment up to 2 years.

Candidate should send CV, cover letter and recommendations to Dr Florence Rivera.

Contacts Dr Florence Rivera

The Laboratory for Photonics and Nanostructures (LPN) is working on an apparatus to measure rate constants of reactions involving biomolecules. A periodic thermal excitation drives the chemical system out-of-equilibrium and kinetic data are extracted from the associated fluorescence response collected in the frequency domain. The technique has been validated relying on oligonucleotides as model reagents and we now aim at proving that its use can be extended to proteins. Three kinds of reactions will be studied: folding, ligand binding, and dimer formation. These demonstration experiments will allow us to advertize our approach to a larger audience and thus they will ease our efforts to develop a commercial product. The present one year project will be realized in the context of a collaboration between the LPN and the Biophysical Chemistry group at the Chemistry Department of the Ecole Normale Supérieure. According to the schedule, some measurements will also be performed on the Disco beamline of the Soleil synchrotron.

Required qualifications:

• PhD in biophysics or biology-related sciences (cell biology, molecular biology) – an equivalent research experience is also acceptable

• Knowledge of thermodynamics and kinetics • Experience in protein expression and purification

• Experience in absorption and fluorescence spectroscopy

• Excellent organization and communication skills

• Ability to collaborate with different teams


Preferable qualifications:

• Experience in fluorescence microscopy

• Experience with fluorescent proteins

• Experience in interfacing and instrumentation

• Background in protein folding, protein-protein interactions


Interested applicants should submit:

• a CV

• some selected publications

• a brief cover letter explaining research experience and interests, as well as career plans

• contact information for three references


Contact : Deadline for applications is April 20th 2015.

Department of Physics & Astronomy “Galileo Galilei” University of Padova (Italy)

Complex fuids fowing under confnement

T h e Surfaces & Interfaces L a b (LaFSI) at the Physics Department of the University of Padova (Italy) announces the opening of an experimental postdoctoral position. The local research activities will be supervised by Dr. M. Pierno and the research project embeds in the framework of the Ideas Starting grant DROEMU (Droplets & Emulsions: Dynamics & Rheology, c.n. 279004). Besides Dr. Pierno, other team members include Prof. G. Mistura (Padua, Italy), Profs. M. Sbragaglia and L. Biferale (Rome, Italy), plus a ten of Post-Docs and PhD students working either in Rome or in Padova. Project: The research activity will experimentally explore the fowing properties of droplets & emulsions confned in microfuidic devices, by means of image based techniques (micro-PIV, image- & specklevelocimetry, etc.). Research topics will focus on droplets dynamics and their collective behaviour. We will address a series of frontier questions related to the manipulation and control of single droplet motion in micro- and nano-channels (e.g. stability & break-up in confned channels) and also focusing on the rheological properties of confned emulsions, to quantify the impact of confnement and boundary conditions on the fow properties and the relationship connecting the shear and stress. Presently the micro-fabrication and fow-imaging facilities are operational both for single (micro)droplets and microfuidic rheology. Throughout all the Research Project the experimental investigation will be accompanied by continuos comparisons with theory and numerical simulations performed in close collaboration with the group of Physics of Complex Fluids at the University of Rome led by Prof. M. Sbragaglia. Position: The position will be activated within June 2015 for 18 months. It is meant for full-time research activity with no teaching load. The gross salary is around 2800 Euros per month. Skills: Experimental skills on microfuidics & microfabrication, optical microscopy and rheology are welcome. Preference will be given to applicants trained in soft-matter, with strong motivations and creativity. A PhD on scientifc disciplines (Physics, Chemistry, Material Science, Chemical Engineering, etc. ) is required. A previous Postdoc experience, even short, is recommended. Interested candidates are pleased to send their CV to


Dr. Matteo Pierno – Department of Physics & Astronomy “Galieo Galilei” Via F. Marzolo 8 – 35131 Padova (PD) Italy

Cadre général

Dans le cadre de l’Institut Pierre Gilles de Gennes pour la Microfluidique (, la Fondation Pierre Gilles de Gennes ( recrute un ingénieur en microfabrication. Affecté(e) à l’IPGG, l’ingénieur recruté(e) collaborera étroitement avec la FPGG, qui est le coordonnateur administratif de l’IPGG.

L’IPGG occupera une surface d’environ 4000m2, située rue Jean Calvin (Paris 5ème). La surface investie comprendra des laboratoires et un plateau technologique mutualisé de 450m2, composé d’une salle blanche de classes ISO 6 et 7 de 100 m2, d’une salle grise de 150 m2, d’une salle de microscopie, d’une salle de culture cellulaire et d’un atelier de micro-usinage.

Cet institut de coopération scientifique est composé de laboratoires appartenant à l’Institut Curie, à l’ESPCI-ParisTech, à l’ENS et à Chimie-ParisTech. Y travaillent des chercheurs et des étudiants des établissements fondateurs, du CNRS et de l’Université Pierre et Marie Curie.



L’ingénieur partagera la responsabilité du parc d’appareils du plateau technologique. Il aura une fonction support pour l’ensemble des personnels de l’institut :

• Supervision des activités de microfabrication

• Etablissement des protocoles

• Formation à l’utilisation des appareils et des technologies

• Conseil et aide au développement de nouveaux protocoles

• Veille technologique

• Participation aux réseaux professionnels associés aux activités technologiques de l’Institut



Le candidat devra être motivé(e), autonome et avoir un esprit collectif. Il aura un intérêt pour la microfluidique, les technologies de soft lithographie et sera attiré(e) par les projets interdisciplinaires.


Les compétences requises pour le poste sont :

• Connaissance approfondie des concepts, techniques et procédés de microfabrication en salle blanche et salle grise.

• Connaissance approfondie des méthodes de caractérisation associées.

• Notions de base en techniques de vide, thermique, optique, mécanique.

• Logiciels de CAO (Ex. Autocad, Catia,…)

• Anglais courant écrit et oral


Des compétences en hygiène et sécurité seront un plus.


Niveau minimal exigé : BAC + 5, master ou équivalent.


Contrat : CDD suivi éventuellement d’un CDI

Salaire : 30-35 k€ bruts annuels selon profil et expérience

Contact pour réponse :


Reference: ABG-60944
Offer type: job

Contract: Contract/Project
Salary range: ≥ 25,000 and < 35,000€ annual gross
Employer: LIMMS/CNRS-University of Tokyo – Institute of Industrial Science
Workplace: Tokyo – JAPAN
Skill area: Biology, Medicine, Health – Biomolecules, pharmacology, therapeutics – Biology, Medicine, Health
Métier : Research and development
Posting date: 2/6/2015
Application deadline: 2/28/2015
The Laboratory for Integrated Micro Mechatronic Systems (LIMMS) is a joint laboratory between the Centre National de la Recherche Scientifique (CNRS) and the Institute of Industrial Science (IIS), the University of Tokyo. It was founded in 1995 to conduct international cooperative research projects in the field of micro-nanomechatronics and was upgraded to UMI (Unité Mixte Internationale) of CNRS as well as an international collaborative research center of IIS. LIMMS has 20 years of experience in international cooperative research and has welcomed more than 150 researchers from France.More information on


2-years position starting in October 2015
Principal investigator at the Institute of Industrial Science (IIS), the University of Tokyo is interested in exploring the challenges and opportunities of Microfluidics in its application to cell/tissue engineering, especially for the cancer including metastasis, angiogenesis , et al. In the experience in microfluidic cell-culture devices they confirmed the potential benefit of microfluidic environment to cell/tissue culture for analytical purposes that could become one of the alternatives to animal testing. They also have developed a couple of key technologies such as, biomaterial science, microtissue units, etc. A new comer to this project is expected to extend this work to advanced microfluidic techniques for cancer cell research. The research from the aspect of mathematical and biophysical/mechanics research fields will be also recommended. The laboratory is well equipped with the apparatus for mammalian cell culture and analysis as well as the full-set of PDMS-based softlithography processes. The project will be in the SMMiL-E initiative framework aiming to promote the fusion between BioMEMS technology of IIS and the structured research against Cancer carried out in SIRIC ONCO-Lille, in France. The research will be done in Japan with close relationship with biology lab and clinician teams in Lille (Centre Oscar Lambret, Lille Biology Institute, U908 INSERM ).

Candidates profile:

Candidates are expected to be skillful in cell culture and biological analysis, and have much interest in microfluidics as well as device fabrication. A challenging sprit would help a lot for tackling into this interdisciplinary and cutting-edge research area.

Please send to limmsadm (at)
with email subject : « LIMMS_PD_2015-2-YOURNAME »
– Your CV (including education & professional history, publication list, reference(s) and photo)
– Letter of Motivation to LIMMS, and the Statement of Purposes (your future plan).
– Selected publication of yours would be helpful

Reference: ABG-60891
Offer type: job

Contract: Contract/Project
Salary range: ≥ 25,000 and < 35,000€ annual gross
Employer: LIMMS/CNRS – University of Tokyo, IIS (Japan)
Workplace: TOKYO – JAPAN
Skill area: Biology, Medicine, Health – Engineering
Métier : Research and development
Posting date: 2/4/2015
Application deadline: 2/28/2015
The Laboratory for Integrated Micro Mechatronic Systems (LIMMS) is a joint laboratory between the Centre National de la Recherche Scientifique (CNRS) and the Institute of Industrial Science (IIS), the University of Tokyo, based in Japan. It was founded in 1995 to conduct international cooperative research projects in the field of micro-nanomechatronics and was upgraded to UMI (Unité Mixte Internationale) of CNRS as well as an international collaborative research center of IIS. LIMMS has 20 years of experience in international cooperative research and has welcomed more than 150 researchers from France.


The 2 year postdoctoral fellowship position will be based in the the Institute of Industrial Science of the University of Tokyo, as a collaboration between Eric Leclerc (LIMMS-Japan, organ on chip), Pr. Sakai (Univ. Tokyo, liver tissue engineering, iPS biology) and Pr. Fujii (Univ. Tokyo, applied microfluidic).

The drug-induced hepatotoxicity (or DILI for Drug-Induced Liver Injury) is a worldwide issue
for clinicians, the pharmaceutical industry and regulatory agencies of the drug. The drug hepatotoxicity is the first acute toxicity leading to death or liver transplantation failure. The hepatotoxicity is also the leading cause of attrition of a large number of therapeutic molecules and their withdrawal from the market. This is the reason why it is essential to develop tools to assess the hepatotoxicity of drug candidates as early as possible during their development. Many models exist at present to estimate the toxicity of drugs (animal models, cell lines, primary hepatocytes). But these models exhibit many limitations, as we will describe later. The project iPS on chip aim to develop, from hepatocytes derived from induced pluripotent stem cells (iPS), a microfluidic culture model that will allow a better stable and reproducible differentiation patterns for assessment xenobiotic toxicity.

The context is the regeneration of tissues and the substitution of bioartificial organs, predictive toxicology, 3D tissue engineering, to overcome the deficiencies of the biological systems, are problems using new multi-disciplinary approaches proposed by Pr Fujii and Pr Sakai. In this frame we propose an integrative research procedure, to improve the iPS differentiation using microfluidic devices.

Research topic
We will need to develop and explore different types of biochips. We will need to optimize the three-dimensional microfluidic biochips suitable for dynamic liver iPS cell culture. For that purpose, BioMEMS recent progress will be implemented in the device conception. It included the consideration of new approach in micro engineering, biomaterials and sensors technology to functionalize the biochips. Then, in this work, we will have to adapt the protocol that allows the cultivation of iPS in the new biochip. To do this, the effects of extracellular matrices, the inoculation density of cells, the co-culture types, the perfusion rates in the biochips, the sequences and doses of additives involved in the differentiation will be tested. We will analyze the pertinence of differentiation in parallel to a Petri. Biological assays to validate the hepatic differentiation will include investigations of mRNA levels analyzed by RTqPCR or microarrays, hepatic biomarkers detection (ALB, AFP), epigenetics analysis and proteomics. For that purpose miniaturization concept and design issues will be important features to allow the best control of endocrine and paracrine factors and the relevant analysis at key time points.

Candidates profile:

Doctor graduate researcher with skills in liver cell biology, stem cell biology, bio engineering, pharmacology

Please send to limmsadm(at) with email subject LIMMS_PD_2015-1-YOURNAME:
– Curriculum vitae (including education & professional history, publication list, reference(s) and photo)
– Motivation letter to LIMMS, and the Statement of Purposes (your future plan).
– One reference and/or recommendation letter